Progress in Synthesis of Water based Solid Phase Peptides

In recent years, green peptide synthesis has become a hot research topic in academia and industry. Traditional solid-state synthesis has gradually exposed problems in the face of the growing demand for green chemistry, mainly in the use of harmful solvents, the use of a large number of washing solvents, excessive reagents and reactants, and the excessive PMI (Process Mass Intensity). The academia and industry have gradually reached a consensus for a long time that green chemical components need to be gradually added in the large-scale peptide drug production process, of which the research progress of green solvent is the fastest. DMF, NMP and DCM used in traditional processes have been gradually noticed by regulators, and large-scale industrial use has been restricted in some countries. In this case, a large number of green solvents, such as cyclopentyl methyl ether (CPEM), methyl tert butyl ether (MTBE), tetrahydrofuran (THF), acetonitrile (ACN), 2-methyltetrahydrofuran (Me THF), ethyl acetate (EtOAc), dimethyl carbonate (DMC) γ- Pentolactone, N-formylmorpholine, and the recently used N-butylpyrrolidone (NBP) have begun to be concerned and used in the solid phase synthesis experiments of different peptides.